Holy Family Hospital serves the Bethlehem region, surrounding villages, and refugee camps operated by the United Nations Relief and Works Agency.  60% of families from Bethlehem have their babies born at Holy Family Hospital, and 25% of those being served reside in refugee camps.  The Hospital serves both the Christian and Muslim communities in the Palestinian Territories.

Holy Family Hospital promotes peace in its interactions with those of other faiths. The Hospital works with officials at the Peres Peace Center in Israel to promote cooperation in the medical field and encourage Israeli-Palestinian interaction.